Event Title

Development of a Sequential Injection Method for On-Line Monitoring of the Maillard Reaction.

Mentor 1

Joseph H. Aldstadt

Location

Union Wisconsin Room

Start Date

24-4-2015 10:30 AM

End Date

24-4-2015 11:45 AM

Description

The Maillard Reaction is a non-enzymatic process involving a series of reactions in which amino acids combine with sugars to form melanoidins. This reaction’s end products have medical implications ranging from hyperglycemia to late diabetic complications. In this study, the early reactions of the Maillard reaction’s kinetics are studied by nuclear magnetic resonance (NMR) spectroscopy. Solutions of phenylalanine methyl ester and methylglyoxal are used as model reactants. A sequential injection (SI) method was developed using a parallel-plate dialyzer (PPD) as a means to separate and preconcentrate the products of the reaction. Development and optimization of the SI-PPD method will be described as well as its interfacing to the NMR spectrometer. Interpretation of the NMR spectra for the Maillard reaction products will be described and implications for the kinetics of the reaction discussed.

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Apr 24th, 10:30 AM Apr 24th, 11:45 AM

Development of a Sequential Injection Method for On-Line Monitoring of the Maillard Reaction.

Union Wisconsin Room

The Maillard Reaction is a non-enzymatic process involving a series of reactions in which amino acids combine with sugars to form melanoidins. This reaction’s end products have medical implications ranging from hyperglycemia to late diabetic complications. In this study, the early reactions of the Maillard reaction’s kinetics are studied by nuclear magnetic resonance (NMR) spectroscopy. Solutions of phenylalanine methyl ester and methylglyoxal are used as model reactants. A sequential injection (SI) method was developed using a parallel-plate dialyzer (PPD) as a means to separate and preconcentrate the products of the reaction. Development and optimization of the SI-PPD method will be described as well as its interfacing to the NMR spectrometer. Interpretation of the NMR spectra for the Maillard reaction products will be described and implications for the kinetics of the reaction discussed.