Optimization of Intermediate Compounds for Direct VDR Binding Assay
Mentor 1
Dr Alexander Arnold
Location
Union Wisconsin Room
Start Date
29-4-2016 1:30 PM
End Date
29-4-2016 3:30 PM
Description
Thousands of secosteroid-based Vitamin D Receptor modulators have been synthesized with a limited number of these being VDR antagonists. This project focuses on the optimization of intermediary compounds in pursuit of a new fluorescent ligand for VDR. The goal was to find the best conditions to return the highest yield for various steps along the synthesis. Column chromatography, thin layer chromatography and optimization of reaction parameters were used to improve the synthesis of the ligand. We were able to significantly increase the yields and improve the separations for each compound. The results create better procedures for the reactions with the hope of maximizing the end product, and providing a more affordable fluorescent VDR ligand for the research world.
Optimization of Intermediate Compounds for Direct VDR Binding Assay
Union Wisconsin Room
Thousands of secosteroid-based Vitamin D Receptor modulators have been synthesized with a limited number of these being VDR antagonists. This project focuses on the optimization of intermediary compounds in pursuit of a new fluorescent ligand for VDR. The goal was to find the best conditions to return the highest yield for various steps along the synthesis. Column chromatography, thin layer chromatography and optimization of reaction parameters were used to improve the synthesis of the ligand. We were able to significantly increase the yields and improve the separations for each compound. The results create better procedures for the reactions with the hope of maximizing the end product, and providing a more affordable fluorescent VDR ligand for the research world.
