Event Title

TCDD Impact on Hematopoietic Stem Cells

Mentor 1

Michael Laiosa

Location

Union Wisconsin Room

Start Date

28-4-2017 1:30 PM

End Date

28-4-2017 4:00 PM

Description

Recent studies provide evidence for the impact of environmental contaminants when exposed to humans/animals cause detrimental damage towards health. In this study we aim to test the impact of TCDD, and the AHR on mitochondrial health in Hematopoietic stem cells (HSCs). Our hypothesis is that AHRs impact on HSC self-renewal has an impact on mitochondrial homeostasis leading to a change in the polarization leading to differentiation within HSC mitochondrial membrane potential. In Vitro cultured bone marrow HSC cells were exposed to TCDD and JC-10 dye in order to identify the impact of TCDD on the mitochondrial health. Our results indicate an impact on the AHR receptor thus changing polarization of mitochondrial health. Our findings indicate, JC-10 optimization has been successful allowing us to now confirm that TCDD impacts JC-10 staining and thus mitochondrial membrane potential. In relation to the three different isolation procedures exposed to JC-10 dye, we can conclude isolation of in vitro culture Hematopoietic Progenitors presented confirmation of polarization within HSC mitochondrial membrane potential. More data is yet to be configured.

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Apr 28th, 1:30 PM Apr 28th, 4:00 PM

TCDD Impact on Hematopoietic Stem Cells

Union Wisconsin Room

Recent studies provide evidence for the impact of environmental contaminants when exposed to humans/animals cause detrimental damage towards health. In this study we aim to test the impact of TCDD, and the AHR on mitochondrial health in Hematopoietic stem cells (HSCs). Our hypothesis is that AHRs impact on HSC self-renewal has an impact on mitochondrial homeostasis leading to a change in the polarization leading to differentiation within HSC mitochondrial membrane potential. In Vitro cultured bone marrow HSC cells were exposed to TCDD and JC-10 dye in order to identify the impact of TCDD on the mitochondrial health. Our results indicate an impact on the AHR receptor thus changing polarization of mitochondrial health. Our findings indicate, JC-10 optimization has been successful allowing us to now confirm that TCDD impacts JC-10 staining and thus mitochondrial membrane potential. In relation to the three different isolation procedures exposed to JC-10 dye, we can conclude isolation of in vitro culture Hematopoietic Progenitors presented confirmation of polarization within HSC mitochondrial membrane potential. More data is yet to be configured.