EVALUATING A DRUG TO BLOCK CELL DEATH IN HUMAN PBMCS
Mentor 1
Chanaka Mendis
Location
Union Wisconsin Room
Start Date
24-4-2015 10:30 AM
End Date
24-4-2015 11:45 AM
Description
In this project, we are looking at Staphylococcus enterotoxin B (SEB) that induces food poisoning and how it affects cell death in the body and how you can terminate it. The first step of this project was to expose different PBMC samples to the drug SP and SB, thereby decreasig the amount of protein that is expected to cause cell death. Then, reverse transcriptase was done to get cDNA. We then ran the cDNA through polymerase chain reaction (PCR). In this step, we took the cDNA and ran each through PCR to amplify the DNA. Then, we ran the proteins through gel electrophoresis to separate them based on size and charge. Then, we quantified the information received from the gel. The ELISA kit was used to look at protein changes. We looked specifically at the Caspase protein that caused cell death in the protein.
EVALUATING A DRUG TO BLOCK CELL DEATH IN HUMAN PBMCS
Union Wisconsin Room
In this project, we are looking at Staphylococcus enterotoxin B (SEB) that induces food poisoning and how it affects cell death in the body and how you can terminate it. The first step of this project was to expose different PBMC samples to the drug SP and SB, thereby decreasig the amount of protein that is expected to cause cell death. Then, reverse transcriptase was done to get cDNA. We then ran the cDNA through polymerase chain reaction (PCR). In this step, we took the cDNA and ran each through PCR to amplify the DNA. Then, we ran the proteins through gel electrophoresis to separate them based on size and charge. Then, we quantified the information received from the gel. The ELISA kit was used to look at protein changes. We looked specifically at the Caspase protein that caused cell death in the protein.
