Gene silencing via microRNA: developing tools for use in an aquatic plant species
Mentor 1
Nicholas Tippery
Mentor 2
Kristen Crossgrove
Location
Union Wisconsin Room
Start Date
24-4-2015 2:30 PM
End Date
24-4-2015 3:45 PM
Description
Gene silencing via microRNA interference (Carbonell et al. (2014)) is a powerful method for investigating gene function. This technique can be used as a way of revealing a function of a gene, and this can help us better understand how to fix a problem such as a disease caused by gene malfunction. Since this technique has been successful in many species, the objective of this experiment is to find out if gene silencing using microRNA interference will work in our species of interest, Nymphoides peltata, an aquatic plant in the family Menyanthaceae. In microRNA inference the gene sequence must be inserted into a plasmid, and transformed into Agrobacterium. This bacteria would then be injected into a plant, and the results would be observed. This technique has already been successful in other species, so I predict that it will be successful in silencing the gene PDS. Turning this gene off would result in loss of pigment (white plant), and its success would mean moving on to other genes of interest in Nymphoides peltata. Results from this experiment would include successfully inserting the gene construct into a plasmid, and being able to transform this into Argrobacterium.
Gene silencing via microRNA: developing tools for use in an aquatic plant species
Union Wisconsin Room
Gene silencing via microRNA interference (Carbonell et al. (2014)) is a powerful method for investigating gene function. This technique can be used as a way of revealing a function of a gene, and this can help us better understand how to fix a problem such as a disease caused by gene malfunction. Since this technique has been successful in many species, the objective of this experiment is to find out if gene silencing using microRNA interference will work in our species of interest, Nymphoides peltata, an aquatic plant in the family Menyanthaceae. In microRNA inference the gene sequence must be inserted into a plasmid, and transformed into Agrobacterium. This bacteria would then be injected into a plant, and the results would be observed. This technique has already been successful in other species, so I predict that it will be successful in silencing the gene PDS. Turning this gene off would result in loss of pigment (white plant), and its success would mean moving on to other genes of interest in Nymphoides peltata. Results from this experiment would include successfully inserting the gene construct into a plasmid, and being able to transform this into Argrobacterium.
