Changes in Expression of AIRAP/AIRAPL in the Presence of Amyloid-Beta as a Protective Measure Against Abeta Toxicity
Mentor 1
Wail Hassan
Location
Union Wisconsin Room
Start Date
28-4-2017 1:30 PM
End Date
28-4-2017 4:00 PM
Description
The aim of this project is to assess the changes in expression of AIRAP/AIRAPL in the presence of Amyloid-Beta (Abeta) to test if the genes are regulated as protective measures against Abeta toxicity. Abeta is thought to play a critical role in the pathogenesis of Alzheimer's disease. The gene that expresses Abeta lies on chromosome 21, patients with Trisomy 21 have an extra copy of this gene and are more prone to developing Alzheimer's at the age of 40. Our study looks at the expression of two genes AIRAP and AIRAPL which help increase degradation of proteins by the proteasome. AIRAP/AIRAPL genes are human homologous to AIP-1 gene in Caenorhaboitis elegans worms. Studies conducted on C.elegans show that AIP-1 has protective effects against Ab toxicity.
To test the changes in AIRAP/AIRAPL expression we will be transfecting WH1 and WH2 cell lines with the Abeta producing gene and GFP as the marker. RNA will then be harvested and the expression of the genes will be measured using qPCR.
Changes in Expression of AIRAP/AIRAPL in the Presence of Amyloid-Beta as a Protective Measure Against Abeta Toxicity
Union Wisconsin Room
The aim of this project is to assess the changes in expression of AIRAP/AIRAPL in the presence of Amyloid-Beta (Abeta) to test if the genes are regulated as protective measures against Abeta toxicity. Abeta is thought to play a critical role in the pathogenesis of Alzheimer's disease. The gene that expresses Abeta lies on chromosome 21, patients with Trisomy 21 have an extra copy of this gene and are more prone to developing Alzheimer's at the age of 40. Our study looks at the expression of two genes AIRAP and AIRAPL which help increase degradation of proteins by the proteasome. AIRAP/AIRAPL genes are human homologous to AIP-1 gene in Caenorhaboitis elegans worms. Studies conducted on C.elegans show that AIP-1 has protective effects against Ab toxicity.
To test the changes in AIRAP/AIRAPL expression we will be transfecting WH1 and WH2 cell lines with the Abeta producing gene and GFP as the marker. RNA will then be harvested and the expression of the genes will be measured using qPCR.
