Event Title

A hand-held optical sensor for bacteria measurement

Presenter Information

Adam Honts

Mentor 1

Marcia Silva

Mentor 2

Rudy Strickler

Location

Union Wisconsin Room

Start Date

27-4-2018 1:00 PM

Description

A hand-held optical sensor for bacteria measurement

HABLE, Basil., University of Wisconsin-Milwaukee; HONTS, Adam., University of Wisconsin-Milwaukee; HANSEN, Thomas., University of Wisconsin-Milwaukee; SILVA, Marcia., University of Wisconsin-Milwaukee, STRICKLER, Rudi, University of Wisconsin-Milwaukee

Corresponding author: Marcia. Silva, msilva@uwm.edu

According to the Environmental Protection Agency (EPA) Method 1603, the current standard method for detecting Escherichia coli (E. coli) bacteria in environmental water, generation of results takes 24 hours. E. coli is a common inhabitant of the intestinal tract and its presence in water is an indication of fecal pollution and possibly enteric pathogens. Therefore, evaluation of the water quality in nearly real time would be ideal for warning bathers. However, this membrane filtration technique is laborious and costly, besides it takes 24 hr for the development of colonies that grow on the surface of a membrane filter and cell counting. This project offers a solution to both the cost effectiveness, temporal, and spatial problems with the current EPA approved method for detecting if E. coli is in a water sample. This is achieved by using digital inline holographic microscopy(DIHM) techniques to produce images of E. coli and reusable materials to keep costs down. Our project also offers the potential of being able to know what kind of bacteria is in the water supply on site. We are currently in the process of compact and portable so that one can take and analyze samples anywhere.

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Apr 27th, 1:00 PM

A hand-held optical sensor for bacteria measurement

Union Wisconsin Room

A hand-held optical sensor for bacteria measurement

HABLE, Basil., University of Wisconsin-Milwaukee; HONTS, Adam., University of Wisconsin-Milwaukee; HANSEN, Thomas., University of Wisconsin-Milwaukee; SILVA, Marcia., University of Wisconsin-Milwaukee, STRICKLER, Rudi, University of Wisconsin-Milwaukee

Corresponding author: Marcia. Silva, msilva@uwm.edu

According to the Environmental Protection Agency (EPA) Method 1603, the current standard method for detecting Escherichia coli (E. coli) bacteria in environmental water, generation of results takes 24 hours. E. coli is a common inhabitant of the intestinal tract and its presence in water is an indication of fecal pollution and possibly enteric pathogens. Therefore, evaluation of the water quality in nearly real time would be ideal for warning bathers. However, this membrane filtration technique is laborious and costly, besides it takes 24 hr for the development of colonies that grow on the surface of a membrane filter and cell counting. This project offers a solution to both the cost effectiveness, temporal, and spatial problems with the current EPA approved method for detecting if E. coli is in a water sample. This is achieved by using digital inline holographic microscopy(DIHM) techniques to produce images of E. coli and reusable materials to keep costs down. Our project also offers the potential of being able to know what kind of bacteria is in the water supply on site. We are currently in the process of compact and portable so that one can take and analyze samples anywhere.