Analysis of how B cells contribute to the Effect of Photobiomodulation Technique in regards to Multiple Sclerosis
Mentor 1
Jeri-Annette Lyons
Location
Union Wisconsin Room
Start Date
27-4-2018 1:00 PM
Description
Multiple Sclerosis is an autoimmune disease that interrupts the flow of information within the brain and between the brain and the body. People who are diagnosed with multiple sclerosis are usually diagnosed between the ages of 20-50. Multiple Sclerosis is more common in women, as disease is two to three times more common in women compared to than men. This study aims to explore how B cells contribute to the effect of photobiomodulation. Photobiomodulation is a therapeutic technique showing promise for the treatment of chronic inflammation and neurodegeneration. This study looks at how different light intensities affect the disease in the absence of B cells by measuring the amount of Interleukin-10 and interferon-gamma. The cells in this study will come from the lymphnodes of mice lacking B cells, which are referred to as the B cell knockout mice. The ELISA technique, using a spectrophotometer, will determine the amount of cytokine in cell culture supernatants. The amount of cytokine, as measured by absorbance of light by the spectrophotometer, is directly proportional to the degree of color change in the assay. The higher the absorbance, the higher the amount of IL-10 or interferon- gamma in the sample. With performing light treatment on the cells and comparing old data to new data, the anticipated results should demonstrate that B cells produce the IL-10 with the application of different intensities of light. The real world significance is that the study can express how the photobiomodulation technique decreases disabilities in Multiple Sclerosis patients, resulting in an improvement in the quality of life.
Analysis of how B cells contribute to the Effect of Photobiomodulation Technique in regards to Multiple Sclerosis
Union Wisconsin Room
Multiple Sclerosis is an autoimmune disease that interrupts the flow of information within the brain and between the brain and the body. People who are diagnosed with multiple sclerosis are usually diagnosed between the ages of 20-50. Multiple Sclerosis is more common in women, as disease is two to three times more common in women compared to than men. This study aims to explore how B cells contribute to the effect of photobiomodulation. Photobiomodulation is a therapeutic technique showing promise for the treatment of chronic inflammation and neurodegeneration. This study looks at how different light intensities affect the disease in the absence of B cells by measuring the amount of Interleukin-10 and interferon-gamma. The cells in this study will come from the lymphnodes of mice lacking B cells, which are referred to as the B cell knockout mice. The ELISA technique, using a spectrophotometer, will determine the amount of cytokine in cell culture supernatants. The amount of cytokine, as measured by absorbance of light by the spectrophotometer, is directly proportional to the degree of color change in the assay. The higher the absorbance, the higher the amount of IL-10 or interferon- gamma in the sample. With performing light treatment on the cells and comparing old data to new data, the anticipated results should demonstrate that B cells produce the IL-10 with the application of different intensities of light. The real world significance is that the study can express how the photobiomodulation technique decreases disabilities in Multiple Sclerosis patients, resulting in an improvement in the quality of life.
