Event Title

Towards a heterologous expression system for hydroxylamine oxidoreductase

Mentor 1

Arsenio Pacheco

Location

Union Wisconsin Room

Start Date

24-4-2015 10:30 AM

End Date

24-4-2015 11:45 AM

Description

Hydroxylamine oxidoreductase (HAO) is an enzyme that ammonia oxidizing bacteria (AOB) use in the process of oxidizing ammonia to nitrite. To date studies of HAO have been hampered by the scarcity of the enzyme, which in turn is due to the slow growth of AOB. To increase the availability of HAO the plan is to genetically modify a fast growing bacterial species so that it will produce HAO in large quantities. HAO is a multi-c-heme protein, a type that is not readily expressed in bacteria such as E. coli, so the long-term plan is to use Shewanella oneidensis to express the protein. Another challenge to the heterologous expression of a protein such as HAO is that it is unclear at present whether such expression requires specialized chaperone proteins. This poster will summarize the initial progress made towards designing an expression system for HAO.

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Apr 24th, 10:30 AM Apr 24th, 11:45 AM

Towards a heterologous expression system for hydroxylamine oxidoreductase

Union Wisconsin Room

Hydroxylamine oxidoreductase (HAO) is an enzyme that ammonia oxidizing bacteria (AOB) use in the process of oxidizing ammonia to nitrite. To date studies of HAO have been hampered by the scarcity of the enzyme, which in turn is due to the slow growth of AOB. To increase the availability of HAO the plan is to genetically modify a fast growing bacterial species so that it will produce HAO in large quantities. HAO is a multi-c-heme protein, a type that is not readily expressed in bacteria such as E. coli, so the long-term plan is to use Shewanella oneidensis to express the protein. Another challenge to the heterologous expression of a protein such as HAO is that it is unclear at present whether such expression requires specialized chaperone proteins. This poster will summarize the initial progress made towards designing an expression system for HAO.