Event Title

Gene silencing via microRNA: developing tools for use in an aquatic plant species

Mentor 1

Nicholas Tippery

Mentor 2

Kristen Crossgrove

Location

Union Wisconsin Room

Start Date

24-4-2015 2:30 PM

End Date

24-4-2015 3:45 PM

Description

Gene silencing via microRNA interference (Carbonell et al. (2014)) is a powerful method for investigating gene function. This technique can be used as a way of revealing a function of a gene, and this can help us better understand how to fix a problem such as a disease caused by gene malfunction. Since this technique has been successful in many species, the objective of this experiment is to find out if gene silencing using microRNA interference will work in our species of interest, Nymphoides peltata, an aquatic plant in the family Menyanthaceae. In microRNA inference the gene sequence must be inserted into a plasmid, and transformed into Agrobacterium. This bacteria would then be injected into a plant, and the results would be observed. This technique has already been successful in other species, so I predict that it will be successful in silencing the gene PDS. Turning this gene off would result in loss of pigment (white plant), and its success would mean moving on to other genes of interest in Nymphoides peltata. Results from this experiment would include successfully inserting the gene construct into a plasmid, and being able to transform this into Argrobacterium.

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Apr 24th, 2:30 PM Apr 24th, 3:45 PM

Gene silencing via microRNA: developing tools for use in an aquatic plant species

Union Wisconsin Room

Gene silencing via microRNA interference (Carbonell et al. (2014)) is a powerful method for investigating gene function. This technique can be used as a way of revealing a function of a gene, and this can help us better understand how to fix a problem such as a disease caused by gene malfunction. Since this technique has been successful in many species, the objective of this experiment is to find out if gene silencing using microRNA interference will work in our species of interest, Nymphoides peltata, an aquatic plant in the family Menyanthaceae. In microRNA inference the gene sequence must be inserted into a plasmid, and transformed into Agrobacterium. This bacteria would then be injected into a plant, and the results would be observed. This technique has already been successful in other species, so I predict that it will be successful in silencing the gene PDS. Turning this gene off would result in loss of pigment (white plant), and its success would mean moving on to other genes of interest in Nymphoides peltata. Results from this experiment would include successfully inserting the gene construct into a plasmid, and being able to transform this into Argrobacterium.