Event Title

Dissection of the Function of ²-Carbonic Anhydrase1 (²-CA1) in HCO3: Transport in Arabidopsis

Mentor 1

Dazhong Zhao

Mentor 2

Jian Huang

Location

Union Wisconsin Room

Start Date

29-4-2016 1:30 PM

End Date

29-4-2016 3:30 PM

Description

The carbonic anhydrases comprise a family of enzymes responsible for catalyzing the interconversion of carbon dioxide and water into bicarbonate and protons, an important process in cellular homeostasis and transport. To identify a possible genetic relationship between certain carbonic anhydrase genes and those of boron, we generated the double mutant bor1-1 β-ca1 and performed phenotypic analysis to establish a connection. This study was performed through the identification of boron transporter and β-carbonic anhydrase mutants in Arabidopsis which were then crossed and analyzed for phenotypic changes. This process included techniques such as DNA extraction, genetic crossing, and PCR analysis. In our results, we found that bor1-1 and ca1 mutants were not defective in anther development. However, the bor1-1 β-ca1 double mutant showed small anthers. At pollen grain mature stage (anther stage 13), part of pollen grains of the bor1-1 β-ca1 double mutant were dead and some of pollen grains showed abnormal large size. This result suggests that β-CA1 and BOR1 are involved in anther development. However, the remaining viable pollen grains are sufficient to support normal reproduction in the bor1-1 β-ca1 mutant. The bor1-1 β-ca1 mutant not displaying a strong defect in anther development may be due to the functional redundancy of the carbonic anhydrase gene family. We will generate ca1 ca2 bor1-1 triple mutant to elucidate how these two gene families affect anther development. These results suggest that regarding the importance of carbonic anhydrase function in cellular homeostasis, the genes involved may have more complex interactions with one another than previously believed.

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Apr 29th, 1:30 PM Apr 29th, 3:30 PM

Dissection of the Function of ²-Carbonic Anhydrase1 (²-CA1) in HCO3: Transport in Arabidopsis

Union Wisconsin Room

The carbonic anhydrases comprise a family of enzymes responsible for catalyzing the interconversion of carbon dioxide and water into bicarbonate and protons, an important process in cellular homeostasis and transport. To identify a possible genetic relationship between certain carbonic anhydrase genes and those of boron, we generated the double mutant bor1-1 β-ca1 and performed phenotypic analysis to establish a connection. This study was performed through the identification of boron transporter and β-carbonic anhydrase mutants in Arabidopsis which were then crossed and analyzed for phenotypic changes. This process included techniques such as DNA extraction, genetic crossing, and PCR analysis. In our results, we found that bor1-1 and ca1 mutants were not defective in anther development. However, the bor1-1 β-ca1 double mutant showed small anthers. At pollen grain mature stage (anther stage 13), part of pollen grains of the bor1-1 β-ca1 double mutant were dead and some of pollen grains showed abnormal large size. This result suggests that β-CA1 and BOR1 are involved in anther development. However, the remaining viable pollen grains are sufficient to support normal reproduction in the bor1-1 β-ca1 mutant. The bor1-1 β-ca1 mutant not displaying a strong defect in anther development may be due to the functional redundancy of the carbonic anhydrase gene family. We will generate ca1 ca2 bor1-1 triple mutant to elucidate how these two gene families affect anther development. These results suggest that regarding the importance of carbonic anhydrase function in cellular homeostasis, the genes involved may have more complex interactions with one another than previously believed.