Date of Award

August 2014

Degree Type

Thesis

Degree Name

Master of Science

Department

Engineering

First Advisor

Mahsa Ranji

Committee Members

Chiu-Tai Law, Habib Tabatabai

Abstract

The structures and biochemistry properties of biological tissues are mostly affected by diseases. The visualization of organ structure and biochemistry helps in early detection and progression monitoring of diseases.

Although, 2D imaging has traditionally been used to gain information from the tissue, it does not accurately represent many of the structures and functions. There currently exists a need for sensitive and specific methods to show detailed information about the structure of the tissue with high resolution and in 3D. The potential advantage of the high resolution 3D images is the ability to accurately probe structural and biochemical properties of the tissue.

Not only the changes in structure, but also the changes in temporal physiological responses affected by oxidative stress (OS) at cellular levels. Thus, it would be valuable to detect the cellular metabolic states, which play a key role in understanding the pathogenesis of the disease, and to develop instruments to detect high resolution 3D images of the tissue.

The objective of this research is to develop a second generation fluorescence optical imaging instrument to image the cellular redox state in 3D, in control and diseases conditions.

I have improved upon one of optical instrument, called cryoimager software and hardware wise to enable higher resolution images. This higher resolution imaging resembles the microscopy capability in cryo temperatures for high resolution 3D imaging.

In conclusion, high resolution optical instrumentation combined with signal and image processing tools provide quantitative physiological and structural information of diseased tissue.

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