Data associated with

Title

Data associated with "Spectrally resolved infrared microscopy and chemometric tools to reveal the interaction between blue light (470 nm) and methicillin-resistant Staphylococcus aureus"

Files

Download 3rd measurement-cube.zip (9.8 MB)

Download 4th Measurement.zip (14.0 MB)

Download 5th measurement.zip (11.0 MB)

Download 6th measurement.zip (22.7 MB)

Download 7th measurement.zip (10.4 MB)

Download 8th measurement.zip (6.2 MB)

Download 9th measurement.zip (13.5 MB)

Download 10th measurement.zip (18.8 MB)

Download 11th measurement.zip (9.0 MB)

Download 12TH measurement.zip (14.6 MB)

Download 2015-08-12-pure vancomycin measurements.zip (12.2 MB)

Download Alive- light.zip (5.7 MB)

Download Alive -Vanco.zip (7.8 MB)

Download alive-vanco-averaging.zip (284 KB)

Download comparison.zip (55.2 MB)

Download dead-vanco.zip (5.7 MB)

Download final trends for mrsa.zip (166.1 MB)

Download MCT data.zip (1.0 MB)

Download mrsa treated.zip (1.9 MB)

Download MRSA_2nd meas-Ctrl vs.Treated-03-23-15-averages.zip (1.7 MB)

Download mrsa_4th.zip (264.6 MB)

Download MRSA_05-06-15.zip (200.2 MB)

Download MRSA_05-28-15.zip (265.3 MB)

Download mrsa_5th.zip (595.2 MB)

Download MRSA_06-03-15.zip (594.2 MB)

Download MRSA_06-18-15.zip (931.4 MB)

Download mrsa_6th.zip (463.8 MB)

Download mrsa_9th meas._06-10-15.zip (594.8 MB)

Download MRSA_12th measurement_07-01-15.zip (957.8 MB)

Download MRSA_alive-alive24h-treated-averages.zip (1.7 MB)

Download mrsa_ctrl.zip (3.9 MB)

Download MRSA-1st meas-alive24h vs. Treated- 02-25-15-averages.zip (1.4 MB)

Download MRSA-2nd meas-Ctrl vs.Treated-03-23-15-cube.zip (107.7 MB)

Download MRSA-04-17-2015.zip (276.4 MB)

Download MRSA-alive-alive24h-treated-cube.zip (2.0 MB)

Download MRSA-very first meas.zip (7.7 MB)

Download ne wmrsa.zip (265.3 MB)

Download NEW MRSA.zip (102.9 MB)

Download averages.tif (89.1 MB)

Download derivative1.tif (88.0 MB)

Download derivative2.tif (88.0 MB)

Download MRSA-1st trial.docx (4.1 MB)

Download Notebook0.txt (4 KB)

Download plot average spectra.pxp (106 KB)

Download ReadMe_N21.pdf (167 KB)

Download Workbook147.xlsx (72 KB)

Description

This data folder is associated with the following publication in the Journal of Photochemistry and Photobbiology: Biology B:

Spectrally resolved infrared microscopy and chemometric tools to reveal the interaction between blue light (470 nm) and methicillin-resistant Staphylococcus aureus

The paper has been published by E. Aboualizadeh, et al. in February 2017. You can acess the paper via the following link:

https://doi.org/10.1016/j.jphotobiol.2016.12.030

When using the data, please cite the publication. Here is the abstract of the publication:

“Blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA), a Gram-positive antibiotic resistant bacterium that leads to fatal infections; however, the mechanism of bacterial death remains unclear. In this paper, to uncover the mechanism underlying the bactericidal effect of blue light, a combination of Fourier transform infrared (FTIR) spectroscopy and chemometric tools is employed to detect the photoreactivity of MRSA and its distinctive pathway toward apoptosis after treatment. The mechanism of action of UV light and vancomycin against MRSA is also investigated to support the findings. Principal component analysis followed by linear discriminant analysis (PCA- LDA) is employed to reveal clustering of five groups of MRSA samples, namely untreated (control I), untreated and incubated at ambient air (control II), irradiated with 470 nm blue light, irradiated with 253.5 UV light, and vancomycin-treated MRSA. Loadings plot from PCA-LDA analysis reveals important functional groups in proteins (1683, 1656, 1596, 1542 cm−1), lipids (1743, 1409 cm−1), and nucleic acids region of the spectrum (1060, 1087 cm−1) that are responsible for the classification of blue light irradiated spectra and control spectra. Cluster vector plots and scores plot reveals that UV light-irradiated spectra are the most biochemically similar to blue light- irradiated spectra; however, some wavenumbers experience a shift. The shifts between blue light and UV light irradiated loadings plot at νasym PO2− band (from 1228 to 1238 cm−1), DNA backbone (from 970 to 966 cm−1) and base pairing vibration of DNA (from 1717 to 1712 cm−1) suggest distinctive changes in DNA conformation in response to irradiation. Our findings indicate that irradiation of MRSA with 470 nm light induces A-DNA cleavage and that B-DNA is more resistant to damage by blue light. Blue light and UV light treatment of MRSA are complementary and distinct from the known antimicrobial effect of vancomycin. Moreover, it is known that UV-induced cleavage of DNA predominantly targets B-DNA, which is in agreement with the FTIR findings. Overall the results suggest that the combination of light and vancomycin could be a more robust approach in treating MRSA infections. “

The data folder contains all the data that has been included in this publication. The data files are stored as .dpt files, which contain FT-IR spectra from alive, blue-light irradiated, UV-irradiated, and vancomycin-treated MRSA.

Please feel free to contact either Dr. Carol Hirschmugl (cjhirsch@uwm.edu) or Dr. Ebrahim Aboualizadeh (eabouali@ur.rochester.edu) for any question or concerns regarding this data.

Publication Date

2017

Disciplines

Physics

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