Event Title

Bacterial expression and purification of proteing in the Met4 transcriptional complex

Mentor 1

Traci Lee

Location

Union Wisconsin Room

Start Date

24-4-2015 10:30 AM

End Date

24-4-2015 11:45 AM

Description

Sulfur metabolism is essential for life, producing the sulfur-containing amino acids methionine and cysteine as well as S-Adenosyl-methionine, the main methyl donor in the cell. We focus on how gene expression is controlled in response to intracellular and extracellular cues in the budding yeast, Saccharomyces cerevisiae. Met4 is a yeast protein that activates the expression of different subsets of sulfur metabolism genes so that cells may appropriately manage its sulfur metabolism in response to different intracellular and extracellular cues. To target the appropriate sulfur metabolism genes for activation, Met4 relies on interactions with DNA-binding proteins, such as Met31 and Met32. Met28 is a protein that is believed to stabilize interactions between Met4 and these proteins. Met31 and Met32 contain very similar

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Apr 24th, 10:30 AM Apr 24th, 11:45 AM

Bacterial expression and purification of proteing in the Met4 transcriptional complex

Union Wisconsin Room

Sulfur metabolism is essential for life, producing the sulfur-containing amino acids methionine and cysteine as well as S-Adenosyl-methionine, the main methyl donor in the cell. We focus on how gene expression is controlled in response to intracellular and extracellular cues in the budding yeast, Saccharomyces cerevisiae. Met4 is a yeast protein that activates the expression of different subsets of sulfur metabolism genes so that cells may appropriately manage its sulfur metabolism in response to different intracellular and extracellular cues. To target the appropriate sulfur metabolism genes for activation, Met4 relies on interactions with DNA-binding proteins, such as Met31 and Met32. Met28 is a protein that is believed to stabilize interactions between Met4 and these proteins. Met31 and Met32 contain very similar