Bacterial expression and purification of proteing in the Met4 transcriptional complex
Mentor 1
Traci Lee
Location
Union Wisconsin Room
Start Date
24-4-2015 10:30 AM
End Date
24-4-2015 11:45 AM
Description
Sulfur metabolism is essential for life, producing the sulfur-containing amino acids methionine and cysteine as well as S-Adenosyl-methionine, the main methyl donor in the cell. We focus on how gene expression is controlled in response to intracellular and extracellular cues in the budding yeast, Saccharomyces cerevisiae. Met4 is a yeast protein that activates the expression of different subsets of sulfur metabolism genes so that cells may appropriately manage its sulfur metabolism in response to different intracellular and extracellular cues. To target the appropriate sulfur metabolism genes for activation, Met4 relies on interactions with DNA-binding proteins, such as Met31 and Met32. Met28 is a protein that is believed to stabilize interactions between Met4 and these proteins. Met31 and Met32 contain very similar
Bacterial expression and purification of proteing in the Met4 transcriptional complex
Union Wisconsin Room
Sulfur metabolism is essential for life, producing the sulfur-containing amino acids methionine and cysteine as well as S-Adenosyl-methionine, the main methyl donor in the cell. We focus on how gene expression is controlled in response to intracellular and extracellular cues in the budding yeast, Saccharomyces cerevisiae. Met4 is a yeast protein that activates the expression of different subsets of sulfur metabolism genes so that cells may appropriately manage its sulfur metabolism in response to different intracellular and extracellular cues. To target the appropriate sulfur metabolism genes for activation, Met4 relies on interactions with DNA-binding proteins, such as Met31 and Met32. Met28 is a protein that is believed to stabilize interactions between Met4 and these proteins. Met31 and Met32 contain very similar
