Event Title

Tomographic Imaging of Exercise- Dependent Adaptations in the Motor Cortex of the Adult Rat

Mentor 1

Rodney Swain

Location

Union 280

Start Date

29-4-2016 12:20 PM

Description

The goal of this research design is to analyze the effects of long-term cerebrovascular changes in the forelimb region of the primary motor cortex in adult rats following exposure to voluntary exercise. 14 Long Evan- Hooded Blue Spruce rats were pseudo- randomly assigned either an inactive control or a voluntary exercise group where the rats were provided full access to a running wheel for twenty- eight days. After the twenty- eight days animals were anesthetized and imaged using spectral domain optical coherence tomography (SD-OCT). Upon finishing the vasculature density, oxygen levels were manipulated using a normoxic condition of 21% oxygen to a hypoxic condition of 10% available oxygen. Between-subject design statistics were employed, using treatment manipulation as the independent variable, along with standard T-tests to determine differences in blood vessel densities. To examine these changes, the brains of control rats and experimental rats were analyzed using SD-OCT. This allowed us to quantify blood vessel density to assess the impact exercise has on the cerebrovascular structure and function. SD-OCT captures high resolution 3-dimensional angiograms, allowing for non-invasive imaging within a single animal at different points in time. To capture the angiograms, cross sections within a 2mm x 2mm scanning field were imaged 10 times before moving onto the next cross-sectional position. Using SD-OCT analysis, blood vessel diameter between the two groups, were able to be quantified using a point count system to provide evidence of exercise- dependent adaptations within the primary motor cortex. We hypothesize that exercise animals will exhibit greater blood vessel density when compared to sedentary controls.

This document is currently not available here.

Share

COinS
 
Apr 29th, 12:20 PM

Tomographic Imaging of Exercise- Dependent Adaptations in the Motor Cortex of the Adult Rat

Union 280

The goal of this research design is to analyze the effects of long-term cerebrovascular changes in the forelimb region of the primary motor cortex in adult rats following exposure to voluntary exercise. 14 Long Evan- Hooded Blue Spruce rats were pseudo- randomly assigned either an inactive control or a voluntary exercise group where the rats were provided full access to a running wheel for twenty- eight days. After the twenty- eight days animals were anesthetized and imaged using spectral domain optical coherence tomography (SD-OCT). Upon finishing the vasculature density, oxygen levels were manipulated using a normoxic condition of 21% oxygen to a hypoxic condition of 10% available oxygen. Between-subject design statistics were employed, using treatment manipulation as the independent variable, along with standard T-tests to determine differences in blood vessel densities. To examine these changes, the brains of control rats and experimental rats were analyzed using SD-OCT. This allowed us to quantify blood vessel density to assess the impact exercise has on the cerebrovascular structure and function. SD-OCT captures high resolution 3-dimensional angiograms, allowing for non-invasive imaging within a single animal at different points in time. To capture the angiograms, cross sections within a 2mm x 2mm scanning field were imaged 10 times before moving onto the next cross-sectional position. Using SD-OCT analysis, blood vessel diameter between the two groups, were able to be quantified using a point count system to provide evidence of exercise- dependent adaptations within the primary motor cortex. We hypothesize that exercise animals will exhibit greater blood vessel density when compared to sedentary controls.