Date of Award
August 2022
Degree Type
Dissertation
Degree Name
Doctor of Philosophy
Department
Biological Sciences
First Advisor
Ching Hong Yang
Committee Members
Sonia Bardy, Sergei Kuchin, Heather Owen, Douglas Steeber
Keywords
c-di-GMP, Dickeya dadantii, diguanylate cyclase, T3SS
Abstract
The enterobacterium, Dickeya dadantii, is an opportunistic bacterial pathogen that causes disease in many plants. C-di-GMP is a ubiquitous bacterial second messenger, regulating multiple cellular behaviors through several c-di- GMP-associated components. Here, we identified a novel transcriptional regulator named CdeR that regulates T3SS in a c-di-GMP-dependent manner. GcpD is a diguanylate cyclase responsible for the synthesis of c-di-GMP. Compared to a gcpD mutant, the gcpD and cdeR double mutant exhibited a reduced T3SS expression. In addition, we found that, under the gcpD mutant background, CdeR regulates T3SS by manipulating intracellular c-di-GMP levels, involving an additional diguanylate cyclase GcpL upregulated by CdeR. This is the first report that uncovers CdeR as a transcriptional regulator involved in the regulation of T3SS. A model is proposed on how CdeR regulates T3SS expression by manipulating the c-di-GMP network.
Recommended Citation
Ghasemimianaei, Alaleh, "A Novel Transcription Factor Cder Regulates the Type III Secretion System in a c-di-GMP Dependent Manner in Dickeya Dadantii" (2022). Theses and Dissertations. 3003.
https://dc.uwm.edu/etd/3003