Data associated with "Understanding the antimicrobial activity of selected disinfectants against methicillin-resistant Staphylococcus aureus (MRSA)"

Authors

Ebrahim Aboualizadeh, University of Wisconsin-MilwaukeeFollow
Violet V. Bumah, University of Wisconsin-Milwaukee
Daniela S. Masson-Meyers, University of Wisconsin-Milwaukee
Janis T. Eells, University of Wisconsin-Milwaukee
Carol J. Hirschmugl, University of Wisconsin-MilwaukeeFollow
Chukuka S. Enwemeka, San Diego State UniversityFollow

Files

Description

This data folder is associated with the following publication in the Journal of Plos One:

Understanding the antimicrobial activity of selected disinfectants against methicillin-resistant Staphylococcus aureus (MRSA)

The paper has been published by E. Aboualizadeh, et al. in October 2017. You can access the paper via the following link:

https://doi.org/10.1371/journal.pone.0186375

When using the data, please cite the publication. Here is the abstract of the publication:

“Disinfectants and biocidal products have been widely used to combat Methicillin-resistant Staphylococcus aureus (MRSA) infections in homes and healthcare environments. Although disruption of cytoplasmic membrane integrity has been documented as the main bactericidal effect of biocides, little is known about the biochemical alterations induced by these chemical agents. In this study, we used Fourier transform infrared (FT-IR) spectroscopy and chemometric tools as an alternative non-destructive technique to determine the bactericidal effects of commonly used disinfectants against MRSA USA-300. FTIR spectroscopy permits a detailed characterization of bacterial reactivity, allowing an understanding of the fundamental mechanism of action involved in the interaction between bacteria and disinfectants. The disinfectants studied were ethanol 70% (N = 5), isopropanol (N = 5), sodium hypochlorite (N = 5), triclosan (N = 5) and triclocarban (N = 5). Results showed less than 5% colony forming units growth of MRSA treated with triclocarban and no growth in the other groups. Nearly 70,000 mid-infrared spectra from the five treatments and the two control (untreated; N = 4) groups of MRSA (bacteria grown in TSB and incubated at 37°C (Control I) / at ambient temperature (Control II), for 24h) were pre-processed and analyzed using principal component analysis followed by linear discriminant analysis (PCA-LDA). Clustering of strains of MRSA belonging to five treatments and the discrimination between each treatment and two control groups in MRSA (untreated) were investigated. PCA-LDA discriminatory frequencies suggested that ethanol-treated spectra are the most similar to isopropanol-treated spectra biochemically. Also reported here are the biochemical alterations in the structure of proteins, lipid membranes, and phosphate groups of MRSA produced by sodium hypochlorite, triclosan, and triclocarban treatments. These findings provide mechanistic information involved in the interaction between MRSA strains and hygiene products; thereby demonstrating the potential of spectroscopic analysis as an objective, robust, and label-free tool for evaluating the macromolecular changes involved in disinfectant-treated MRSA."

The data folder contains all the data that has been included in this publication. The data files are stored as .dpt files in the order of dates that we collected data. Dpt files contain FT-IR spectra from alive and five different disinfectant-treated MRSA as well as FT-IR spectra from pure disinfectants studied in this publication. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. All relevant data are within the paper.

Please feel free to contact either Dr. Carol Hirschmugl (cjhirsch@uwm.edu) or Dr. Ebrahim Aboualizadeh (eabouali@ur.rochester.edu) for any question or concerns regarding this data.

Publication Date

10-16-2017

Disciplines

Physics

Recommended Citation

Aboualizadeh E, Bumah VV, Masson-Meyers DS, Eells JT, Hirschmugl CJ, Enwemeka CS (2017) Understanding the antimicrobial activity of selected disinfectants against methicillin-resistant Staphylococcus aureus (MRSA). PLoS ONE 12(10): e0186375. https://doi.org/10.1371/journal.pone.0186375