Map Based Cloning of Five Male-Sterility Genes in Soybean
Mentor 1
Dr. Devinder Sandhu
Location
Union Wisconsin Room
Start Date
24-4-2015 2:30 PM
End Date
24-4-2015 3:45 PM
Description
Sterility refers to spores and gametes that are abnormal or absent, or individuals that fail to produce functional gametes to produce offspring. Since manual cross-pollination to produce large quantities of hybrid soybean seed is difficult and time consuming, identifying a stable male-sterile system would create a breakthrough in hybrid seed production. Five male-sterile soybean mutants (ms1, ms4, ms5, ms6 and ms8) have been identified and mapped. Objectives of this investigation were to fine map and clone these five genes controlling male fertility in soybean. Genetic linkage mapping was used to locate ms1, ms4, ms5, ms6 and ms8 genes to chromosomes 13, 2, 11, 13 and 7, respectively. The comparisons of the genetic linkage maps with the sequence based physical maps helped in localizing ms1, ms4, ms5, ms6 and ms8 to 76, 216, 1988, 1142 and 160 Kb regions, respectively. Predicted genes in the corresponding regions were studied for the predicted functions and candidate genes were identified for each of the male-sterility genes. We are currently sequencing wild type and mutant alleles to identify causes of mutants. Characterization of the genes involved in male fertility may play a critical role in development of economically feasible soybean hybrids. /
Map Based Cloning of Five Male-Sterility Genes in Soybean
Union Wisconsin Room
Sterility refers to spores and gametes that are abnormal or absent, or individuals that fail to produce functional gametes to produce offspring. Since manual cross-pollination to produce large quantities of hybrid soybean seed is difficult and time consuming, identifying a stable male-sterile system would create a breakthrough in hybrid seed production. Five male-sterile soybean mutants (ms1, ms4, ms5, ms6 and ms8) have been identified and mapped. Objectives of this investigation were to fine map and clone these five genes controlling male fertility in soybean. Genetic linkage mapping was used to locate ms1, ms4, ms5, ms6 and ms8 genes to chromosomes 13, 2, 11, 13 and 7, respectively. The comparisons of the genetic linkage maps with the sequence based physical maps helped in localizing ms1, ms4, ms5, ms6 and ms8 to 76, 216, 1988, 1142 and 160 Kb regions, respectively. Predicted genes in the corresponding regions were studied for the predicted functions and candidate genes were identified for each of the male-sterility genes. We are currently sequencing wild type and mutant alleles to identify causes of mutants. Characterization of the genes involved in male fertility may play a critical role in development of economically feasible soybean hybrids. /
