Antibody Purification Using Protein Hydrogels
Mentor 1
Ionel Popa
Location
Union Wisconsin Room
Start Date
28-4-2017 1:30 PM
End Date
28-4-2017 4:00 PM
Description
Antibody purification via protein binding has been extensively used within the biotechnology industry. Protein L is a protein commonly used in the purification of antibodies. It binds to the variable light-chain of an antibody, thus having a wider range of antibodies it can bind when compared to other proteins. Here we demonstrate how protein L(8) can be cross-linked and polymerized into a hydrogel and how this hydrogel can bind, retain and release IgG antibodies in a highly controlled manner. By altering the concentration of protein L we can affect the binding capacity. This property of protein hydrogels may provide a new potential method for the purification of antibodies.
Antibody Purification Using Protein Hydrogels
Union Wisconsin Room
Antibody purification via protein binding has been extensively used within the biotechnology industry. Protein L is a protein commonly used in the purification of antibodies. It binds to the variable light-chain of an antibody, thus having a wider range of antibodies it can bind when compared to other proteins. Here we demonstrate how protein L(8) can be cross-linked and polymerized into a hydrogel and how this hydrogel can bind, retain and release IgG antibodies in a highly controlled manner. By altering the concentration of protein L we can affect the binding capacity. This property of protein hydrogels may provide a new potential method for the purification of antibodies.
