Identification of Basal Tissue Folds in the Developing Gastrointestinal Tract of the Early Vertebrate Embryo Authors; Thomas Stancato, Mike Visetsouk, Jennifer Gutzman
Mentor 1
Jennifer Gutzman
Location
Union Wisconsin Room
Start Date
5-4-2019 1:30 PM
End Date
5-4-2019 3:30 PM
Description
In order for organs to function properly, they must undergo structural changes during development, and without proper structure, disease can occur. We first discovered basal tissue folding and basal constriction in the highly conserved midbrain-hindbrain boundary (MHB). After discovering mechanisms that mediate this tissue fold, we hypothesized that other organs also undergo basal tissue folding and basal constriction in order to change their structure. Research shows that during early vertebrate development, the gut tube and neural tube that gives rise to the brain and spinal cord share similar structural characteristics. Both the neuroepithelium and the intestinal epithelium are lined on their basal side by a basement membrane. The epithelial sheets in the gut give rise to villi which are anchored to the basement membrane. We hypothesize these villi undergo basal folding and constriction similar to the MHB. Using the zebrafish as a model organism, we have initiated our investigation into the potential basal folding in this gut tube. By labeling cells with phalloidin and labeling the basement membrane with fluorescent laminin antibodies, we imaged larvae with confocal microscopy. Our preliminary data suggest that basal folding occurs during villi formation. We are currently performing experiments using Green Fluorescent Protein (GFP) to live image larvae intestinal epithelium at single-cell resolution in order to examine cell shape, particularly basal constriction.
Identification of Basal Tissue Folds in the Developing Gastrointestinal Tract of the Early Vertebrate Embryo Authors; Thomas Stancato, Mike Visetsouk, Jennifer Gutzman
Union Wisconsin Room
In order for organs to function properly, they must undergo structural changes during development, and without proper structure, disease can occur. We first discovered basal tissue folding and basal constriction in the highly conserved midbrain-hindbrain boundary (MHB). After discovering mechanisms that mediate this tissue fold, we hypothesized that other organs also undergo basal tissue folding and basal constriction in order to change their structure. Research shows that during early vertebrate development, the gut tube and neural tube that gives rise to the brain and spinal cord share similar structural characteristics. Both the neuroepithelium and the intestinal epithelium are lined on their basal side by a basement membrane. The epithelial sheets in the gut give rise to villi which are anchored to the basement membrane. We hypothesize these villi undergo basal folding and constriction similar to the MHB. Using the zebrafish as a model organism, we have initiated our investigation into the potential basal folding in this gut tube. By labeling cells with phalloidin and labeling the basement membrane with fluorescent laminin antibodies, we imaged larvae with confocal microscopy. Our preliminary data suggest that basal folding occurs during villi formation. We are currently performing experiments using Green Fluorescent Protein (GFP) to live image larvae intestinal epithelium at single-cell resolution in order to examine cell shape, particularly basal constriction.
