3D Culture of Patient-Derived Xenograft (PDX) Mammary Organoids
Mentor 1
Qingsu Cheng
Start Date
28-4-2023 12:00 AM
Description
Three-dimensional (3D) culture models is an emerging technology that is more relevant to study the initiation, progression, and metastasis of breast cancer. 3D culture models can recapitulate in vitro environments more accurately and decipher the complex cell-cell and cell-matrix interactions that separate normal and malignant tissues. 3D culture models of normal malignant breast epithelial cells are essential for advancing the understanding of breast cancer progression and developing more effective treatments. Compared to the traditional two-dimensional (2D) culture systems, 3D cultures more closely mimic the complex tissue architecture and cellular interactions found in vivo; therefore, 3D cultures offer a more accurate representation of cell behavior and drug response. Moreover, patient-derived xenograft (PDX) models are one more step towards better representing the complexity and heterogeneity of human tumors in comparison to traditional cancer cell lines. Since PDX models retain the genetic and histological features of the original patient tumor, making them a more accurate representation of the patient’s disease. Additionally, PDX models have shown great value in predicting patient responses to therapy, which makes them invaluable for preclinical drug testing and personalized medicine. In this undergraduate research, we propose to use confocal and structural illumination microscopies for verifying research results and comparing them to existing literature. By utilizing advanced imaging techniques, we can bring a more accurate understanding of breast cancer and its underlying mechanisms. The success of this proposed research is measured by comparing the obtained results to the existing literature. If the results are validated, we will continue our research on the topic of screening drugs.
3D Culture of Patient-Derived Xenograft (PDX) Mammary Organoids
Three-dimensional (3D) culture models is an emerging technology that is more relevant to study the initiation, progression, and metastasis of breast cancer. 3D culture models can recapitulate in vitro environments more accurately and decipher the complex cell-cell and cell-matrix interactions that separate normal and malignant tissues. 3D culture models of normal malignant breast epithelial cells are essential for advancing the understanding of breast cancer progression and developing more effective treatments. Compared to the traditional two-dimensional (2D) culture systems, 3D cultures more closely mimic the complex tissue architecture and cellular interactions found in vivo; therefore, 3D cultures offer a more accurate representation of cell behavior and drug response. Moreover, patient-derived xenograft (PDX) models are one more step towards better representing the complexity and heterogeneity of human tumors in comparison to traditional cancer cell lines. Since PDX models retain the genetic and histological features of the original patient tumor, making them a more accurate representation of the patient’s disease. Additionally, PDX models have shown great value in predicting patient responses to therapy, which makes them invaluable for preclinical drug testing and personalized medicine. In this undergraduate research, we propose to use confocal and structural illumination microscopies for verifying research results and comparing them to existing literature. By utilizing advanced imaging techniques, we can bring a more accurate understanding of breast cancer and its underlying mechanisms. The success of this proposed research is measured by comparing the obtained results to the existing literature. If the results are validated, we will continue our research on the topic of screening drugs.
