Cabin1 is vital for growth and survival of larvae zebrafish
Mentor 1
Ava Udvadia
Location
Union Wisconsin Room
Start Date
27-4-2018 1:00 PM
Description
DiGeorge syndrome is a pediatric disorder in which patients with multigene deletions on the long arm of chromosome 22 present with craniofacial deformities in addition to a host of other symptoms. We have developed a zebrafish model to test the function of Calcineurin binding protein 1(Cabin1), a gene found within Chromosome 22 deletions in a subset of DiGeorgepatients. In our previous studies, reduced expression of Cabin1 was observed to result in craniofacial deformities and reduced survival (Hammond-Weinberger, 2012). We have since generated zebrafish with CRISPR/Cas9-targeted mutation Cabin1, to more comprehensively investigate the effect of Cabin1 gene knockout on development. Based on our previous studies demonstrating jaw defects with reduced Cabin1 expression, we hypothesize that Cabin1 deletion mutants will have reduced growth and survival. Here we present the results of a blinded 30-day survival study to compare survival between the wild-type and Cabin1 mutant zebrafish over a period of thirty days. Each day, we documented survival, eating behaviors, swimming patterns, and morphological development of the fish. The length of surviving zebrafish was measured at the termination of the experiment to determine the effects of the Cabin1 mutation on growth. Our initial experiments support our hypothesis and we are currently testing a second Cabin1 mutant strain for reproducibility.
Cabin1 is vital for growth and survival of larvae zebrafish
Union Wisconsin Room
DiGeorge syndrome is a pediatric disorder in which patients with multigene deletions on the long arm of chromosome 22 present with craniofacial deformities in addition to a host of other symptoms. We have developed a zebrafish model to test the function of Calcineurin binding protein 1(Cabin1), a gene found within Chromosome 22 deletions in a subset of DiGeorgepatients. In our previous studies, reduced expression of Cabin1 was observed to result in craniofacial deformities and reduced survival (Hammond-Weinberger, 2012). We have since generated zebrafish with CRISPR/Cas9-targeted mutation Cabin1, to more comprehensively investigate the effect of Cabin1 gene knockout on development. Based on our previous studies demonstrating jaw defects with reduced Cabin1 expression, we hypothesize that Cabin1 deletion mutants will have reduced growth and survival. Here we present the results of a blinded 30-day survival study to compare survival between the wild-type and Cabin1 mutant zebrafish over a period of thirty days. Each day, we documented survival, eating behaviors, swimming patterns, and morphological development of the fish. The length of surviving zebrafish was measured at the termination of the experiment to determine the effects of the Cabin1 mutation on growth. Our initial experiments support our hypothesis and we are currently testing a second Cabin1 mutant strain for reproducibility.
